Mme GRAZON Chloé

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Téléphone 05 40 00 25 21
Statut Permanent
Poste Chercheur
Batiment A12
Etage 3° Ouest

Research interests

I develop nanobiophotonic probes, i.e. nanoparticles that interact with light and are able to sense events at the biological level, for bioanalytics and bioimaging. My current research includes the design, synthesis,functionalization and spectroscopic characterization of ultrabright NPs (polymer or pure dyes). My main project is currently the ERC COMET.

  •  Fluorescent nanoparticles (organics: FONs, polymers ; or inorganics: quantum dots)
  •  FRET
  •  Biosensors
  •  Colloïds
  •  Surface functionalization (with DNA, proteins...)
  •  Polymer synthesis

Grants / Fundings

  •  2023-2028: ERC St COMET
  •  2023-2026: ANR JCJC SensET (declined)
  •  2022 : CNRS MITI FONcoustics. Collaboration with Dr B. Arnal, LiPhy, Grenoble, FR.
  •  2022 : CNRS PEPS COFLUO. Collaboration with Dr G. Le Fer, UMET, Lille, FR.
  •  2021: young scientist starting grant from the Materials and Light Sciences Department of Bordeaux Univ.
  •  2017-2020 : Marie Curie global fellowship H2020 SENSHOR between Boston University & Université de Bordeaux

Collaborations (past & present)

  • M. W. Grinstaff    Boston U., USA    
  • B. Arnal    LiPhy, FR    
  • G. Le Fer    UMET, FR    
  • F. Gauffre    ISCR, FR    
  • G. Gouget    ISCR, FR    
  • A. Estevez-Thorres    LASIRE, FR
  • F. Perez    Curie Institute, FR    
  • Y. Medina-gonzalez    LOF, FR    
  • S. Lecommandoux    LCPO, FR    
  • C. Bonduelle    LCPO, FR    
  • E. Garanger    LCPO, FR    
  • E. Cloutet    LCPO, FR    
  • A.-T. Bui    ISM, FR    
  • P. Loubet    ISM, FR
  • Withings, FR

Institutional responsabilities, ISM

  •  co-founder with P. Loubet of the laboratory's environmental footprint team
  •  member of the institute board

Journal covers

covers_grazon

 

 

 

 

 

 

 

Other personal pages

 - ORCID
 - Google Scholar
 - LinkedIn

Publications

Estevez-Torres, A., Gauffre, F., Gouget, G., Grazon, C., Loubet, P. (). Carbon footprint and mitigation strategies of three chemistry laboratories. In Green Chemistry (Vol. 26, Issue 5, p. 2613-2622). https://doi.org/10.1039/d3gc03668e
Yuan, X., Yang, K., Grazon, C., Wang, C., Vallan, L., Isasa, J.D., Resende, P.M., Li, F., Brochon, C., Remita, H., Hadziioannou, G., Cloutet, E., Li, J. (). Tuning the Aggregates of Thiophene-based Trimers by Methyl Side-chain Engineering for Photocatalytic Hydrogen Evolution. In Angewandte Chemie - International Edition (Vol. 63, Issue 1, p. e202315333). https://doi.org/10.1002/anie.202315333
Beauseroy, H., Grazon, C., Antoine, S., Badreldin, M., Salas-Ambrosio, P., Harrisson, S., Garanger, E., Lecommandoux, S., Bonduelle, C. (). Polypeptide- and Protein-Based Conjugate Nanoparticles via Aqueous Ring-Opening Polymerization-Induced Self-Assembly (ROPISA). In Macromolecular Rapid Communications. https://doi.org/10.1002/marc.202400079
Grazon, C., Garanger, E., Lalanne, P., Ibarboure, E., Galagan, J.E., Grinstaff, M.W., Lecommandoux, S. (). Transcription-Factor-Induced Aggregation of Biomimetic Oligonucleotide-b-Protein Micelles. In Biomacromolecules (Vol. 24, Issue 11, p. 5027-5034). https://doi.org/10.1021/acs.biomac.3c00662
Yuan, X., Wang, C., Vallan, L., Bui, A.T., Jonusauskas, G., McClenaghan, N.D., Grazon, C., Lacomme, S., Brochon, C., Remita, H., Hadziioannou, G., Cloutet, E. (). Organic Conjugated Trimers with Donor–Acceptor–Donor Structures for Photocatalytic Hydrogen Generation Application. In Advanced Functional Materials (Vol. 33, Issue 15, p. 2211730). https://doi.org/10.1002/adfm.202211730
Pan, M., Cruz, G.M., Grazon, C., Kechkeche, D., Renault, L.H., Clavier, G., Méallet-Renault, R. (). Luminescence-Sensitive Surfaces Bearing Ratiometric Nanoparticles for Bacteria Growth Detection. In ACS Applied Polymer Materials (Vol. 4, Issue 8, p. 5482-5492). https://doi.org/10.1021/acsapm.2c00549
Si, Y., Grazon, C., Clavier, G., Audibert, J.F., Sclavi, B., Méallet-Renault, R. (). FRET-mediated quenching of BODIPY fluorescent nanoparticles by methylene blue and its application to bacterial imaging. In Photochemical and Photobiological Sciences (Vol. 21, Issue 7, p. 1249-1255). https://doi.org/10.1007/s43630-022-00215-1
Grazon, C., Chern, M., Lally, P., Baer, R.C., Fan, A., Lecommandoux, S., Klapperich, C., Dennis, A.M., Galagan, J.E., Grinstaff, M.W. (). The quantum dot vs. organic dye conundrum for ratiometric FRET-based biosensors: which one would you chose?. In Chemical Science (Vol. 13, Issue 22, p. 6715-6731). https://doi.org/10.1039/d1sc06921g
Sankar, K., Baer, R., Grazon, C., Sabatelle, R.C., Lecommandoux, S., Klapperich, C.M., Galagan, J.E., Grinstaff, M.W. (). An Allosteric Transcription Factor DNA-Binding Electrochemical Biosensor for Progesterone. In ACS Sensors (Vol. 7, Issue 4, p. 1132-1137). https://doi.org/10.1021/acssensors.2c00133
Grazon, C., Salas-Ambrosio, P., Antoine, S., Ibarboure, E., Sandre, O., Clulow, A.J., Boyd, B.J., Grinstaff, M.W., Lecommandoux, S., Bonduelle, C. (). Aqueous ROPISA of α-amino acid: N -carboxyanhydrides: Polypeptide block secondary structure controls nanoparticle shape anisotropy. In Polymer Chemistry (Vol. 12, Issue 43, p. 6242-6251). https://doi.org/10.1039/d1py00995h
Grazon, C., Baer, R.C., Kuzmanović, U., Nguyen, T., Chen, M., Zamani, M., Chern, M., Aquino, P., Zhang, X., Lecommandoux, S., Fan, A., Cabodi, M., Klapperich, C., Grinstaff, M.W., Dennis, A.M., Galagan, J.E. (). A progesterone biosensor derived from microbial screening. In Nature Communications (Vol. 11, Issue 1, p. 1276). https://doi.org/10.1038/s41467-020-14942-5
Chen, M., Grazon, C., Sensharma, P., Nguyen, T.T., Feng, Y., Chern, M., Baer, R.C., Varongchayakul, N., Cook, K., Lecommandoux, S., Klapperich, C.M., Galagan, J.E., Dennis, A.M., Grinstaff, M.W. (). Hydrogel-embedded quantum dot−transcription factor sensors for quantitative progesterone detection. In ACS Applied Materials and Interfaces (Vol. 12, Issue 39, p. 43513-43521). https://doi.org/10.1021/acsami.0c13489
Si, Y., Grazon, C., Clavier, G., Rieger, J., Tian, Y., Audibert, J.F., Sclavi, B., Méallet-Renault, R. (). Fluorescent Copolymers for Bacterial Bioimaging and Viability Detection. In ACS Sensors (Vol. 5, Issue 9, p. 2843-2851). https://doi.org/10.1021/acssensors.0c00981
Chen, M., Nguyen, T.T., Varongchayakul, N., Grazon, C., Chern, M., Baer, R.C., Lecommandoux, S., Klapperich, C.M., Galagan, J.E., Dennis, A.M., Grinstaff, M.W. (). Surface Immobilized Nucleic Acid–Transcription Factor Quantum Dots for Biosensing. In Advanced Healthcare Materials (Vol. 9, Issue 17, p. 2000403). https://doi.org/10.1002/adhm.202000403
Grazon, C., Salas-Ambrosio, P., Ibarboure, E., Buol, A., Garanger, E., Grinstaff, M.W., Lecommandoux, S., Bonduelle, C. (). Aqueous Ring-Opening Polymerization-Induced Self-Assembly (ROPISA) of N-Carboxyanhydrides. In Angewandte Chemie - International Edition (Vol. 59, Issue 2, p. 622-626). https://doi.org/10.1002/anie.201912028
Chern, M., Grazon, C., Dennis, A.M. (). Phase Transfer and DNA Functionalization of Quantum Dots Using an Easy-to-Prepare, Low-Cost Zwitterionic Polymer. In Methods in Molecular Biology (Vol. 2135, p. 125-139). https://doi.org/10.1007/978-1-0716-0463-2_7
Grazon, C., Si, Y., Placial, J.P., Rieger, J., Méallet-Renault, R., Clavier, G. (). Core-shell polymeric nanoparticles comprising BODIPY and fluorescein as ultra-bright ratiometric fluorescent pH sensors. In Photochemical and Photobiological Sciences (Vol. 18, Issue 5, p. 1156-1165). https://doi.org/10.1039/c8pp00457a
Nguyen, T.T., Chern, M., Baer, R.C., Fan, A., Grazon, C., Galagan, J., Dennis, A.M. (). QD-FRET-based biosensing of small molecule analytes using transcription factor-DNA binding. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 10892, p. 108920Q). https://doi.org/10.1117/12.2516576
Grazon, C., Chern, M., Ward, K., Lecommandoux, S., Grinstaff, M.W., Dennis, A.M. (). A versatile and accessible polymer coating for functionalizable zwitterionic quantum dots with high DNA grafting efficiency. In Chemical Communications (Vol. 55, Issue 74, p. 11067-11070). https://doi.org/10.1039/c9cc04856a
Kechkeche, D., Cao, E., Grazon, C., Caschera, F., Noireaux, V., Baron Niel, M.L., Dubertret, B. (). Semiconductor Nanoplatelets: A New Class of Ultrabright Fluorescent Probes for Cytometric and Imaging Applications. In ACS Applied Materials and Interfaces (Vol. 10, Issue 29, p. 24739-24749). https://doi.org/10.1021/acsami.8b07143
Banerjee, A., Grazon, C., Pons, T., Bhatia, D., Valades-Cruz, C.A., Johannes, L., Krishnan, Y., Dubertret, B. (). A novel type of quantum dot-transferrin conjugate using DNA hybridization mimics intracellular recycling of endogenous transferrin. In Nanoscale (Vol. 9, Issue 40, p. 15453-15460). https://doi.org/10.1039/c7nr05838a
Bhatia, D., Arumugam, S., Nasilowski, M., Joshi, H., Wunder, C., Chambon, V., Prakash, V., Grazon, C., Nadal, B., Maiti, P.K., Johannes, L., Dubertret, B., Krishnan, Y. (). Quantum dot-loaded monofunctionalized DNA icosahedra for single-particle tracking of endocytic pathways. In Nature Nanotechnology (Vol. 11, Issue 12, p. 1112-1119). https://doi.org/10.1038/nnano.2016.150
Grazon, C., Rieger, J., Beaunier, P., Méallet-Renault, R., Clavier, G. (). Fluorescent core-shell nanoparticles and nanocapsules using comb-like macromolecular RAFT agents: Synthesis and functionalization thereof. In Polymer Chemistry (Vol. 7, Issue 25, p. 4272-4283). https://doi.org/10.1039/c6py00646a
Grazon, C., Rieger, J., Beaunier, P., Méallet-Renault, R., Clavier, G. (). Erratum: Fluorescent core-shell nanoparticles and nanocapsules using comb-like macromolecular RAFT agents: Synthesis and functionalization thereof (Polymer Chemistry (2016) DOI: 10.1039/c6py00646a). In Polymer Chemistry (Vol. 7, Issue 25, p. 4284). https://doi.org/10.1039/c6py90091g
Si, Y., Grazon, C., Clavier, G., Rieger, J., Audibert, J.F., Sclavi, B., Méallet-Renault, R. (). Rapid and accurate detection of Escherichia coli growth by fluorescent pH-sensitive organic nanoparticles for high-throughput screening applications. In Biosensors and Bioelectronics (Vol. 75, p. 320-327). https://doi.org/10.1016/j.bios.2015.08.028
Banerjee, A., Grazon, C., Nadal, B., Pons, T., Krishnan, Y., Dubertret, B. (). Fast, Efficient, and Stable Conjugation of Multiple DNA Strands on Colloidal Quantum Dots. In Bioconjugate Chemistry (Vol. 26, Issue 8, p. 1582-1589). https://doi.org/10.1021/acs.bioconjchem.5b00221
Grazon, C., Rieger, J., Charleux, B., Clavier, G., Méallet-Renault, R. (). Ultrabright BODIPY-tagged polystyrene nanoparticles: Study of concentration effect on photophysical properties. In Journal of Physical Chemistry C (Vol. 118, Issue 25, p. 13945-13952). https://doi.org/10.1021/jp502790w
Grazon, C., Rieger, J., Méallet-Renault, R., Charleux, B., Clavier, G. (). Ultrabright fluorescent polymeric nanoparticles made from a new family of BODIPY monomers. In Macromolecules (Vol. 46, Issue 13, p. 5167-5176). https://doi.org/10.1021/ma400590q
Grazon, C., Rieger, J., Méallet-Renault, R., Clavier, G., Charleux, B. (). One-pot synthesis of pegylated fluorescent nanoparticles by RAFT miniemulsion polymerization using a phase inversion process. In Macromolecular Rapid Communications (Vol. 32, Issue 9-10, p. 699-705). https://doi.org/10.1002/marc.201100008
Grazon, C., Rieger, J., Sanson, N., Charleux, B. (). Study of poly(N,N-diethylacrylamide) nanogel formation by aqueous dispersion polymerization of N,N-diethylacrylamide in the presence of poly(ethylene oxide)-b-poly(N,N-dimethylacrylamide) amphiphilic macromolecular RAFT agents. In Soft Matter (Vol. 7, Issue 7, p. 3482-3490). https://doi.org/10.1039/c0sm01181a
Rieger, J., Grazon, C., Charleux, B., Alaimo, D., Jérôme, C. (). Pegylated thermally responsive block copolymer micelles and nanogels via in situ RAFT aqueous dispersion polymerization. In Journal of Polymer Science, Part A: Polymer Chemistry (Vol. 47, Issue 9, p. 2373-2390). https://doi.org/10.1002/pola.23329